Hieff™ Bst Plus DNA Polymerase (40 U/μL) _ 14402ES

Yeasen - Leading Innovation in Molecular Enzymes and Reagents Verified independent store

→

Hieff™ Bst Plus DNA Polymerase is derived from Thermophilic Geobacillus sp DNA Polymerase I, lacking 5'-3' exonuclease activity. The enzyme has stronger 5'-3' DNA polymerase activi ty, strand displacement activity, and dUTP tolerance, which is more suitable for anti-pollution isothermal amplification reactions, such as LAMP, CPA, etc. Features Thermophilic DNA polymerase with strong strand displacement activity Applications Isothermal DNA amplification Specifications Polymerase Bst DNA Polymerase Heat Inactivation Incubation at 85°C for 5 min. Solution Components 10 mmol/L Tris-HCl, 50 mmol/L KCl, 0.1 mmol/L EDTA, 1 mmol/L DTT, 0.1% Triton X-100, 50% Glycerol, pH 7.5 @ 25℃. Activity Definition 1 U refers to the amount of enzyme required to incorporate 10 nmol of dNTP into the acid-insoluble precipitate in 30 min at 65°C. Components Components No. Name 14402ES92 (8,000 U) 14402ES97(40,000 U) 14402-A Hieff™ Bst Plus DNA Polymerase (40 U/μL) 200 μL 1 mL 14402-B 10 ×Hieff™ Bst Plus DNA Polymerase Buffer 1 mL 3×1 mL 14402-C 100 mmol/L MgSO4 1 mL 3×1 mL Figures High Sensitivity Figure 1: Sensitivity Test Red: 50 copies/T, response less than 15 minutesFigure 1: The copy numbers of 50 copies/Tweres tested. The test results showed that within 15 minutes, the results of 50 copies/T performed better, and the detection rate reached 100%. Stability Figure 2.Stability testing at the same temperature Red: 37℃ 0 day Blue: 37℃ 1 day Green: 37℃ 3 days Purple: 37℃ 5 days0 days at 37°C as a control and stability tests were carried out for 1, 3, and 5 days at the same temperature, indicating that it can still maintain a relatively stable effect at 37°C for 5 days. Figure 3. -20℃ repeated freeze-thaw stability test Red: Repeat freeze-thaw 0 timesBlue: Repeat freeze-thaw 5 timesGreen: Repeat freeze-thaw 10 times Specificity Figure 4. 48-well NTC-specific assay 48-well NTC detection was used in the specific detection of the product, and no peak was detected, indicating that the specificity was better. Storage The product should be stored at -25℃ ~ -15℃ for 2 years. Please avoid repeated freeze-thaw. Documents: Safety Data Sheet 14402_MSDS_HB251125 Manuals 14402_Manual_HB230115 Related Blogs: What is the role of Bst DNA polymerase in LAMP? High-quality Bst Enzyme Lyophilized Material Makes Molecular POCT More Convenient Show more