phi29 DNA polymerase is the replicative polymerase from Bacillus subtilis bacteriophage, has exceptional strand displacement and processive synthesis properties. It can synthesize DNA fragments up to 70 kb. The polymerase has an inherent 3´→5' proofreading exonuclease activity. And it is recommended to modify the 3 'end of the primer in the system to prevent degradation. phi29 DNA polymerase is often used for plasmid synthesis in vitro and whole genome synthesis. Features High sensitivity: It is capable of effectively amplifying DNA templates at the pg level. High amplification yield: When 100 ng of template is input, the yield can reach 2 μg/μL. Applications DNA Enzymatic Synthesis. Whole genome amplification (WGA). Rolling circle amplification (RCA). Multiplex displacement amplification (MDA). SNP detection. Specifications Unit Definition One unit is defined as the amount of enzyme that will incorporate 0.5 pmol of dNTP into acid insoluble material in 10 minutes at 30°C. Heat Inactivation 65°C for 10 min Components Components No. Name 14404ES72 14404ES80 14404ES90 14404ES92 14404-A phi29 DNA Polymerase (10 U/μL） 25 μL 100 μL 500 μL 1 mL 14404-B 10×phi29 Reaction Buffer 100 μL 400 μL 2×1 mL 4×1 mL Shipping and Storage This product should be stored at -25 ~ -15oC for 2 years. Figures Figure 1.Whole genome amplification with different DNA template input amounts using phi29 DNA polymerases from Yeasen and Supplier A . C1: No template control, C2: No enzyme control. The results showed that phi29 DNA polymerase (Cat#14404) had better sensitivity and amplification yield than the competitor's product. Figure 2.The amplification yield of Yeasen phi29 DNA polymerase. Template: 293 cell gDNA, with an input of 100 ng; Reaction time: 16 h. The results showed that the amplification yield of Yeasen phi29 DNA polymerase (Cat#14404) could reach 2 μg/μL when the input amount of template was 100 ng. Documents: Safety Data Sheet 14404_MSDS_Ver.EN20250113.pdf Manuals 14404_Manual_Ver.EN20240513.pdf
