Exonuclease III (E. coli) is a 3′ -> 5′ exonuclease which acts by digesting one strand of a dsDNA duplex at a time or digesting the RNA strand of an RNA-DNA heteroduplex. Exonuclease III (E. coli) breaks phosphodiester bonds on the 5′-side of AP sites in both dsDNA and ssDNA. It removes 3′-terminal groups on dsDNA, increases MutY turnover, and efficiently degrades 3′-recessed but not 3′ protruding DNA ends (creating 5′-overhangs). Exonuclease III (E. coli) removes a limited number of nucleotides per binding event, resulting in coordinated progressive deletions within the population of DNA molecules. Applications Degrades excess single-stranded primer oligonucleotides from a reaction mixture containing double-stranded extension products Components Components No. Name 14525ES90 (5 KU) 14525ES96 (25 KU) 14525-A Exonuclease III (10 U/µL) 50 μL 5x 50 μL 14525-B 10 x Exo III Buffer 500 μL 5x 500 μL Shipping and Storage The products should be stored at -25℃ ~ -15℃ for 2 years. Documents: Safety Data Sheet 14525_MSDS_HB250211 Manuals 14525_Manual_Ver.EN20250723
