Endonuclease IV can act on a variety of oxidative damage in DNA. The enzyme is apurinic/apyrimidinic (AP) endonuclease that will hydrolyse intact AP sites in DNA. AP sites are cleaved at the first phosphodiester bond that is 5´ to the lesion leaving a hydroxyl group at the 3´ terminus and a deoxyribose 5´-phosphate at the 5´ terminus. The enzyme also has a 3´ -diesterease activity and can release phosphoglycolaldehyde, intact deoxyribose 5-phosphate and phosphate from the 3´ end of DNA. Applications Single cell gel electrophoresis (Comet assay) Alkaline elution Alkaline unwinding Specifications Source E.coli Molecular Weight 32.5 KD Concentration 10 U/μL Unit Definition One unit is defined as the amount of enzyme required to cleave 1 pmol of a 34-mer oligonucleotide duplex containing a single AP site in a total reaction volume of 10 µl in 1 hour at 37°C. An AP site is created by treating 10 pmol of a 34 mer oligonucleotide duplex containing a single uracil residue with 1 unit of Uracil-DNA Glycosylase (UDG) for 2 minutes at 37°C. Components Components No. Name 14543ES80 14543ES90 14543-A Endonuclease IV(10 U/μL) 100 μL 500 μL 14543-B 10 x Endonuclease IV Buffer 0.8 mL 1.25 mL Shipping and Storage This product should be stored at-25~-15℃ for 1 years. Documents: Safety Data Sheet 14543_MSDS_Ver.EN20250121 Manuals 14543_Manual _Ver.CN20250121.pdf
