The FuniCutTM series of restriction endonucleases are rapidly acting, genetically engineered enzymes capable of precisely cutting DNA within 5 to 15 minutes. They are ideal for the rapid digestion of plasmid DNA, PCR products, genomic DNA, and more. The FuniCutTM series shares a universal digestion buffer, simplifying the reaction setup, and offers excellent enzyme activity redundancy, making it easy to handle substrate excess or challenging templates. Specifications Enzyme SmaI Recognition Site 5'-CCC↓GGG-3' 3'-GGG↑CCC-5' Recommended Reaction Conditions 1× FuniCutTM Buffer; incubate at 37°C. Enzyme Activity 20 U/μL Inactivation Conditions Incubate at 80°C for 20 minutes. Components Components No. Name 15027ES60 15027-A FuniCutTM SmaI 100 μL 15027-B 10×FuniCutTM Buffer 1 mL 15027-C 10×FuniCutTM Color Buffer* 1 mL [Note]:*10xFuniCutTM Color Buffer includes red and yellow tracking dyes, allowing direct use of the product for gel electrophoresis. The red dye migrates similarly to a 2500 bp double-stranded DNA fragment in 1% agarose gel; the yellow dye migrates similarly to a 10 bp double-stranded DNA fragment in 1% agarose gel. Shipping and Storage This product should be stored at -25℃~-15℃ for up to 2 years. Documents: Safety Data Sheet 15027_MSDS_HB250801_EN.PDF Manuals 15027_Manual_Ver.EN20250728.pdf
