Hieff™ Flash Cell /Tissue Total RNA Kit was performed by using Hieff DNA removing / RNA binding column technology and new solution systems suitable for extracting high purity and quality total RNA from a variety of fresh or frozen animal tissues or cultured cells. The extraction process does not require toxic phenol, chloroform, β -mercaptoethanol, or precipitation of isopropanol and ethanol. Easy to operate, 15 min to complete the animal tissue or cells (10-30 mg animal tissue or (1-10) ×106 Animal cells) of total RNA extraction. The total RNA is of high purity and can be used in various molecular biology experiments including RT-PCR, qPCR, molecular cloning and RNase protection analysis. Features Ultra-Fast: RNA extraction in under 15 minutes per sample. High RNA Integrity: Proprietary buffer formulation preserves full-length RNA throughout the workflow. Broad Sample Compatibility: Works efficiently with challenging samples, including primary cells, protein-rich muscle tissue, lipid-rich brain tissue, and aquatic species (e.g., fish and shrimp). Effective gDNA Removal: Integrated DNA-removal/RNA-binding spin column eliminates genomic DNA contamination in just 30 seconds—no DNase treatment required. Components No. Name 19221ES50 (50 T) 19221-A Hieff DNA removing/RNA binding Column A 100 19221-B 2 mL Collection Tube A2 100 19221-C Lysate buffer LB（LB Buffer A2） 30 mL 19221-D Deproteinized buffer PL (PL Buffer A 2) 40 mL 19221-E Wash Buffer W*(Wash Buffer A 2*) 13 mL 19221-F RNase-free H2O 5 mL Figures Figure 1. High RNA integrity across diverse tissues with Yeasen Kit (Cat. No. 19221). RNA was extracted from liver (P-1, P-2), muscle (H-1, H-2), kidney (H-3, H-4), spleen (H-5), brain (H-6), skin (fH-4), shrimp, loach, and crab meat. All samples show clear 28S and 18S rRNA bands with a ~2:1 ratio, indicating excellent RNA integrity.
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