This Kit is designed for rapid and efficient RNA extraction from Gram-positive and Gram-negative bacteria.Utilizing a proprietary silica- membrane column technology combined with optimized buffers, this kit effectively removes contaminants such as proteins, metabolites, and genomic DNA. The purified RNA is suitable for downstream applications including RT-PCR, RT-qPCR, in vitro transcription, and molecular cloning. Specifications Cat.No. 19301ES50 Size 50 T Components Category Components No. Name 19301ES50 Part I 19301-A Lysozyme 20 mg Part II 19301-B DNA-Removing Column B4 50 units 19301-C Hieff™ RNA Column B4 50 units 19301-D 2 mL Collection Tube B4 100 units 19301-E Lysis Buffer(LB Buffer B4) 25 mL 19301-F PL Buffer B4 40 mL 19301-G Wash Buffer B4* 13 mL(add 52 mL ethanol before use) 19301-H TE Buffer pH 8.0 6 mL 19301-I RNase-free H2O 5 mL Features Ø Wide applicability: can be used for bacterial cultures such as Escherichia coli, Pseudomonas aeruginosa, and Gram-positive bacteria. Ø Quick and easy: the operation can be completed within 40 minutes. Ø High RNA purity: DNase I on-column digestion effectively removes gDNA. Ø Safe and environmentally friendly: no need to use organic matter such as phenol and chloroform for extraction. Applications Bacterial RNA extraction；RNA extraction from Gram-positive bacteria；Gram-negative bacteria, etc. Shipping and Storage Part I components should be stored at 4~8℃ for 2 years. Part II components should be stored at room temperature for 2 years. Figures Suppliers Concentration OD260/OD280 OD260/OD230 Yeasen-19031ES 159.40/193.18 1.895/1.925 2.177/2.101 Supplier T* 182.01/179.31 1.855/1.922 0.728/1.206 Table 1. E. coli extract product concentration Figure 1. Electrophoresis of E. coli RNA extraction products Take 4 portions of 2 mL of E. coli suspension, use 19301 and similar products from supplier T brand to extract total RNA, and elute with 50 ul RNase-free H2O. Then take 1 ul of the eluate to determine the concentration. Take 5 ul of the eluate to identify the RNA integrity and band brightness. The results show that the 19301 extraction product has a high yield and good RNA integrity. Documents: Safety Data Sheet 19301_MSDS_HB250407 Manuals 19301_Manual_Ver.EN20250825 Citations & References: [1] Ma X, Hou M, Liu C, Li J, Ba Q, Wang H. Cadmium accelerates bacterial oleic acid production to promote fat accumulation in Caenorhabditis elegans. J Hazard Mater. 2022;421:126723. doi:10.1016/j.jhazmat.2021.126723(IF:10.588) [2] Wang Y, Hu W, Deng Z, He X. Rapid identification of magnesium ascorbyl phosphate utilizing phosphatase through a chromogenic change-coupled activity assay. Appl Microbiol Biotechnol. 2021;105(7):2901-2909. doi:10.1007/s00253-021-11229-7(IF:4.813)
