The Enhanced ECL Chemiluminescent Substrate Kit is designed for the detection of horseradish peroxidase (HRP)-labeled primary or secondary antibodies and their associated antigens. The principle involves separating proteins or nucleic acids by electrophoresis, transferring them onto a blotting membrane, and then incubating with a primary antibody followed by an HRP-conjugated secondary antibody, or using an HRP-labeled probe to directly or indirectly bind target nucleic acids on the membrane. After washing, the membrane is incubated at room temperature for several minutes with freshly prepared ECL working solution from this kit. The membrane is then wrapped in plastic wrap, placed face-up into an X-ray film cassette, and exposed to X-ray film in a darkroom for seconds to hours. Following development and fixation, protein or nucleic acid bands are clearly visualized on the X-ray film. This kit features a unique luminescent substrate system that reduces background signal while incorporating a novel oxidant to significantly enhance stability, allowing the reagent to remain stable for one year at room temperature. In addition to X-ray film detection, the chemiluminescent signal can be directly captured using a fluorescence CCD imager. This product is primarily intended for Western blot (WB) applications and chemiluminescent immunoassay systems. Features Superb Sensitivity: Detects antigens at picogram to low femtogram levels for ultra-sensitive Western blot results. High Signal-to-Noise Ratio: Advanced luminescent substrates deliver strong signals with minimal background noise. Antibody-Efficient: Optimized formulation enhances antibody binding, reducing antibody consumption. Excellent Value: Delivers top-tier performance at a competitive price. Outstanding Stability: Improved oxidant system ensures stable storage at 4°C for up to 12 months. Specifications Applicable Samples Target with high abundance, routine protein samples Detection Sensitivity Medium picogram level Signal Duration < 2 h Applicable Detection System All reagents are suitable for X-ray film, CCD imaging devices Primary Antibody Dilution Concentration 1:1000-1:4000 Secondary Antibody Dilution Concentration 1:1000-1:4000 Recommended Membrane NC membrane Storage Stability Room temperature for 1 year Advantages Strongest signal among the same level of sensitivity Components Components No. Name 36222ES60 36222ES76 36222-A Solution A 50 mL 250 mL 36222-B Solution B 50 mL 250 mL Application Chemiluminescence ELISA Western Blot Dot Blot-DNA/RNA Southern blot-DNA Northern blot-RNA
