Hieff Trans™ mRNA Transfection Reagent is a cationic polymer transfection reagent specifically designed for mRNA cellular transfection, which exhibits high transfection efficiency for routine cells, primary cells, and neural cells. Its unique formulation allows it to be directly added to the culture medium without the serum affecting the transfection efficiency, thus reducing the damage to cells caused by serum removal. There is no need to remove nucleic acid-transfection reagent complexes or change to fresh culture medium after transfection, and fresh culture medium can also be replaced based on the nutritional status of the cells after 4-6 hours. Features Stable and Highly Soluble: Colorless and clear; forms RNA-based nanoparticles that remain stable at room temperature for at least 30 minutes, ensuring reproducible experiments and consistent results. Broad Compatibility: Functions effectively across pH 4–8 and is well-tolerated in physiological buffers and serum, enhancing transfection efficiency in complex environments. Versatile Applications: Performs excellently with mRNA, siRNA, and gene editing tools, achieving up to 95% gene editing efficiency in vitro. User-Friendly: Compatible with standard laboratory conditions, requiring no specialized equipment and simplifying experimental procedures. Applications mRNA drug development Gene function research CRISPR/Cas9 gene editing Specifications Form liquid Nucleic Acid mRNA/siRNA/miRNA/ASO Category In vitro Cell Primary cells/ Immune cells/ Epithelial cells and other cells. Components Components No. Name 40809ES01 40809ES03 40809 Hieff Trans™ mRNA Transfection Reagent 0.1 mL 1 mL Figures High-Efficiency mRNA Transfection Figure 1. A parallel comparison experiment of EGFP mRNA delivery and expression in different cell lines, showing superior performance to competitor T in a variety of cell lines.
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