Kanamycin sulfate is an aminoglycoside antibiotic that has inhibitory effects on both Gram-positive and Gram-negative bacteria, as well as mycoplasmas. Its mechanism of action targets the binding to the bacterial 70S ribosomal subunits, thereby inhibiting ribosomal translocation and causing misreading, which further interferes with protein synthesis. Kanamycin sulfate can be used in cell culture to suppress bacterial contamination. In molecular biology research, it is commonly used for selective screening of bacterial colonies that have successfully been transformed with the kanamycin resistance gene (kan gene); it is also often used in Agrobacterium-mediated transformation experiments for the selective screening of plant tissues carrying the npt II (APH(3')-II) gene. This product is easily soluble in water (100 mg/mL), and it should be filtered and sterilized before use. The recommended working concentration is 30-100 µg/mL. For other antibiotics, please refer to the related topic of Antibiotic User Manual. Features Potency (dry basis) ≥750 µg/mg Standardized production, using factory mass production mode Applications Cell culture Commonly used to selectively screen bacterial colonies Frequently used in Agrobacterium-mediated transformation experiments to selectively screen plant tissues carrying the npt II (APH3) gene Specifications CAS NO. 25389-94-0 Molecular Fomular C18H36N4O11·H2O4S Molecular Weight 582.58 g/mol Appearance White or off-white Potency (dry basis) ≥ 750 µg/mg Structure Components Components No. Name 60206ES10 60206ES60 60206 Kanamycin Sulfate 10 g 100 g Shipping and Storage The Kanamycin Sulfate products be stored at room temperature for 3 years. Documents: Safety Data Sheet 60206_MSDS_HB250210_EN.pdf Manuals 60206_Manual_HB250210_EN.pdf
