Product Name Cat# Size Recombinant Human IL-21 Protein 90215ES05 2 μg 90215ES10 10 μg 90215ES50 50 μg 90215ES60 100 μg 90215ES80 1 mg IL-21 is a pleiotropic cytokine produced by CD4+ T cells in response to antigenic stimulation, and its effects typically enhance the antigen-specific response of immune cells.The biological effects of IL-21 include: inducing T cell-stimulated B cells to differentiate into plasma cells and memory B cells; combining with IL-4 to stimulate IgG production; and, in the absence of T cell signaling, inducing apoptotic effects in naïve B cells and stimulated B cells to induce apoptotic effects. In addition, IL-21 promotes the antitumor activity of CD8+ T cells and NK cells.IL-21 exerts its effects by binding to a specific type I cytokine receptor, IL-21R, which also contains the gamma chain (γc) found in other cytokine receptors, including IL-2, IL-4, IL-7, IL-9, and IL-15.IL-21/IL -21R interaction triggers a cascade of events, including activation of the tyrosine kinases JAK1 and JAK3, followed by activation of the transcription factors STAT1 and STAT3. This product, Recombinant Human IL-21, is supplied as a lyophilized powder with high activity, high purity and low endotoxin. Specification Synonyms Interleukin-21; IL21; Za11 Source E.coli-derived human IL-21 protein, Gln30-Ser162 without tag. Accession Q9HBE4 Molecular Weight Approximately 15.4 kDa. AA Sequence QGQDRHMIRM RQLIDIVDQL KNYVNDLVPE FLPAPEDVET NCEWSAFSCF QKAQLKSANT GNNERIINVS IKKLKRKPPS TNAGRRQKHR LTCPSCDSYE KKPPKEFLER FKSLLQKMIH QHLSSRTHGS EDS Endotoxin < 1.0 EU/μg by the LAL method. Purity > 97% by SDS-PAGE and HPLC. Biological Activity Fully biologically active when compared to standard. Measured by its ability to enhance IFN-gamma secretion in NK-92 human natural killer lymphoma cells. The ED50 for this effect is ≤ 2 ng/mL. The specific activity of recombinant human IL-21 > 5.0 × 105 U/mg. Formulation Lyophilized from a 0.2 µm filtered concentrated solution in PBS, pH 7.4. Physical Appearance White lyophilized powder Storage -25 ~ -15 ° C storage, valid for 1 year after receipt. After re-solution, store at 2 ~8 °C, 30 days expiration date. After re-dissolving, store at -85~-65°C, 3 months expiration date. Compounding Methods Centrifuge before uncapping to bring contents to the bottom. Reconstitute in sterile distilled water to a concentration of 0.1-1.0 mg/mL, which can be diluted even further according to subsequent experiments; for long-term storage, it is recommended that carrier proteins (0.1% BSA) be added to the dilution buffer; dispense for a single experimental dose and freeze at -80°C to avoid repeated freezing and thawing. Cautions 1. Please operate with lab coats and disposable gloves,for your safety. 2. This product is for research use only.
