Interleukin-3 (IL-3) is a hematopoietic growth factor that promotes the survival, differentiation, and proliferation of directed progenitor cells of the megakaryocyte, granulocyte-macrophage, erythroid, eosinophil, basophil, and mast cell lineages.IL-3 is produced by T-cells, mast cells, and eosinophils, and enhances thrombopoietin, phagocytosis, and antibody mediated cytotoxicity. Its ability to activate monocytes suggests that IL-3 may have additional immunomodulatory roles. Much IL-3 activity is dependent on co-stimulation with other cytokines.IL-3 is a species-specific, variably glycosylated cytokine. Recombinant Human IL-3 is a 15.0 kDa globular protein containing 133 amino acid residues. This Recombinant Human IL-3 is supplied as Lyophilized powder with high activity, high purity, low endotoxin, and NO labeling. Product Properties Synonyms Interleukin-3, IL-3, Hematopoietic growth factor, MCGF, Multipotential colony-stimulating factor, P-cell-stimulating factor Species Human Source CHO Cells Sequence Ala20-Phe152 Accession P08700 Tag NO Molecular Weight Predicted molecularmass：15 kDa Endotoxin ≤10 EU/mg by the LAL method Purity ≥95%, by SDS-PAGE (under reducing (R) & Non-reducing conditions, visualized by Coomassie staining) Activity Measured in a cell proliferation assay using TF-1 human erythroleukemiccells. The ED50 for this effect is 0.02-0.1 ng/mL Formulation Lyophilized from a 0.22 μm-filtered solution containing PBS, 5% mannitoland 0.01% Tween 80, pH 7.4 Appearance Lyophilized powder Shipping and Storage -25 ~ -15 ° C storage, from the date of receipt of goods, the expiration date of 1 year. After re-solution, store at 2 ~8 °C, 7-10 days expiration date. After re-dissolution, -85 ~ -65 ° C storage, 6 months expiration date. Compounding method Use NO bacterial deionized water to reconstitute lyophilized proteins. It is recommended that proteins be divided for first use to avoid repeated freeze-thawing. Figure Figure 1.Measured in a cell proliferation assay using TF-1 human erythroleukemiccells. The ED50 for this effect is 0.02-0.1 ng/mL. Figure 2.Recombinant Human IL-3 SDS-PAGE under reducing (R) condition. Cautions 1. For your safety and health, please wear lab coat and disposable gloves. 2. This product is for scientific research purposes only.
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