Interleukin-4 (IL-4) is a cytokine that belongs to the interleukin family. Interleukin 4 is mainly produced by T cells, B cells, macrophages and other immune cells.IL4 is a key regulatory molecule that plays an important role in the Th2 type of immune response. Interleukin 4 has a variety of biological functions that promote B cell class switching to IgE and IgG1, thereby enhancing the effects of humoral immunity. In addition, IL4 inhibits the activation of macrophages and dendritic cells and suppresses their release of inflammatory cytokines. In clinical practice, interleukin 4 is widely used in the treatment of allergic diseases, such as asthma and allergic rhinitis. This product, Recombinant Human 4, is supplied as Lyophilized powder with high activity, high purity and low endotoxin. Product Properties Synonyms Interleukin-4, IL4, BSF-1, BCDF, IL4E12, Pitrakinra Species Human Source CHO Cells Sequence His25-Ser153 Accession P05112 Tag NO Molecular Weight Predicted molecularmass：15 kDa Endotoxin ≤10 EU/mg by the LAL method Purity ≥95%, by SDS-PAGE (under reducing (R) & Non-reducing conditions, visualized by Coomassie staining) Activity Measured in a cell proliferation assay using TF-1 cells, The ED50 for thiseffect is ≤ 0.2 ng/mL，orresponding to a specific activity of ＞ 1x 107 units/mg. Formulation Lyophilized from a 0.22 μm-filtered solution containing PBS, 5% mannitoland 0.01% Tween 80, pH 7.4 Appearance Lyophilized powder Shipping and Storage -25 ~ -15 ° C storage, from the date of receipt of goods, the expiration date of 1 year. After re-solution, store at 2 ~8 °C, 7-10 days expiration date. After re-dissolution, -85 ~ -65 ° C storage, 6 months expiration date. Compounding method Use NO bacterial deionized water to reconstitute lyophilized proteins. It is recommended that proteins be divided for first use to avoid repeated freeze-thawing. Figure Figure 1.Measured in a cell proliferation assay using TF-1 cells, The ED50 for this effect is ≤ 0.2 ng/mL，Corresponding to a specific activity of ＞ 1 x 10^7 units/mg. Figure 2.Recombinant Human IL-4 SDS-PAGE under reducing (R) condition. Cautions 1. For your safety and health, please wear lab coat and disposable gloves. 2. This product is for scientific research purposes only.
