PDGF is a disulfide-linked dimer consisting of two 12.0-13.5 kDa polypeptide chains, named PDGF-A and PDGF-B. PDGF is stored in platelet α-granules and released upon platelet activation. PDGF is involved in many biological processes, including proliferation, chemotaxis, embryonic neuronal development, and respiratory tubule epithelial cell development. Use sterile deionized water to reconstitute the freeze-dried protein. It is recommended to divide the protein into aliquots when using it for the first time to avoid repeated freezing and thawing. Features Recombinant human PDGF-AA derived from CHO cells. Provided in the form of lyophilized powder. High activity, high purity. Low endotoxin. No labeling. Applications The three naturally occurring PDGF-AA, PDGF-BB, and PDGF-AB are potent mitogens for a variety of cell types, including smooth muscle cells, connective tissue cells, bone and cartilage cells, and some blood cells. Specifications Synonyms Fibroblast Growth Factor-4, HST-1, Transforming protein KS3, HBGF-4 Species Human Source CHO Cells Sequence Ser87-Thr211 Accession P04085 Tag None Molecular Weight Predicted molecular mass：14.3kDa(monomer) Endotoxin ≤10 EU/mg by the LAL method Purity ≥95%, by SDS-PAGE (under reducing (R) & Non-reducing conditions, visualized by Coomassie staining) Activity The activity is determined by the dose-dependent proliferation of NIH3T3 cells is typically between ≤5.0 ng/mL. Formulation Lyophilized from a 0.22 μm-filtered solution containing PBS, 5% mannitol and 0.01% Tween 80, pH 7.4 Appearance Lyophilized powder Components Components No. Name 91612ES10 91612ES10 91612ES80 91612 Recombinant Human PDGF-AA Protein(CHO-expressed) 10 μg 50 μg 1 mg Shipping and Storage Store at -25~-15℃, valid for 1 year from the date of receipt. After reconstitution, store at 2~8°C, valid for 7-10 days. After reconstitution, store at -85~-65℃, valid for 6 months. Documents:
