Hepatocyte Growth Factor(HGF), also known as Scatter Factor and Hepatopoietin A, is a pleiotropic protein in the Plasminogen subfamily of S1 peptidases. It is a multidomain molecule that includes an N-terminal PAN/APPLE-like domain, four Kringle domains, and a serine proteinase-like domain that has no detectable protease activity (1 - 5). Mouse HGF is secreted as an inactive 728 amino acid (aa) single chain propeptide. It is cleaved after the fourth Kringle domain by a serine protease to form bioactive disulfide-linked HGF with a 60 kDa alpha and 30 kDa beta chain. Specification Synonyms F-TCF; hepatocyte growth factor (hepapoietin A; scatter factor); Hepatopoeitin-A; Hepatopoietin A; Uniprot No. P08048 Source Human HGF Protein is expressed from HEK293 without tag. It contains Gln33-Arg495 (alpha)&Vla496-Leu728(beta). Molecular Weight The protein has a predicted 79.3 kDa Purity > 95% as determined by SDS-PAGE. Biological Activity Measured in a cell proliferation assay using mIMCD-3 mouse epithelial cells. The EC50 for this effect is 1-10 ng/mL. Endotoxin < 0.01 EU per 1 μg of the protein by the LAL method. Formulation Dissolved in sterile PBS buffer. Concentration 0.2 mg/ml Storage The product should be stored at -85~-65℃ for 1 year from date of receipt. Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles. Caution 1.Please operate with lab coats and disposable gloves,for your safety. 2.This product is for research use only.
