Cynomolgus IL-3 R alpha/CD123 Protein, His Tag

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Specifications Synonyms CD123; IL3RA; IL3R; IL3RAY Source Cynomolgus IL-3 R alpha/CD123 Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Arg18-Arg302.[A ccession | G8F3K3-1] Molecular Weight The protein has a predicted MW of 33.9 kDa. Due to glycosylation, the protein migrates to 50-70 kDa based on Tris-Bis PAGE result. Endotoxin Less than 1EU per μg by the LAL method. Purity > 95% as determined by SDS-PAGE and HPLC. Activity SPR Data: Human IL-3, hFc Tag captured on CM5 Chip via Protein A can bind Cynomolgus IL-3 R alpha, His Tag with an affinity constant of 0.46 μM as determined in SPR assay (QC Test).Cynomolgus IL-3 R alpha, His Tag immobilized on CM5 Chip can bind Cynomolgus IL-3, His Tag with an affinity constant of 1.30 nM as determined in SPR assay . Formulation Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. Storage The product should be stored at -25~-15℃ for 1 year from date of receipt. 2-7 days, 2 ~ 8 °C under sterile conditions after reconstitution. 3 -6 months, -85~-65℃ under sterile conditions after reconstitution. Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles. Note 1.Please operate with lab coats and disposable gloves,for your safety. 2.This product is for research use only. Show more