Product Properties Alternative Names Interleukin-8, IL-8, GCP-1, MDNCF, MONAP, NAP-1, GCP/IL-8 protein IV, IL8/NAP1 form I, CXCL8, LECT, LUCT, LYNAP, NAF Species Cynomolgus Expression Sequence Ala23-Pro100 Accession A0A2K5TUL7 Tag C-His Expression System HEK293 Molecular Weight The protein has a predicted MW of 10.12 kDa. Due to glycosylation, the protein migrates to 12-14 kDa based on SDS-PAGE result. Purity > 95% as determined by SDS-PAGE. Endotoxin < 1.0 EU per μg by the LAL method. Formulation Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Appearance Lyophilized Powder Biological Activity ELISA Data: Immobilized Cynomolgus IL-8, His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Anti-IL-8 Antibody, hFc Tag with the EC50 of 13.0ng/ml determined by ELISA. Reconstitution Method Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. Features High purity, high activity, low endotoxin, high batch-to-batch consistency Storage Store at -25 to -15°C. Once received, the product is valid for one year. After resuspension, store at -85 to -65°C. The product is valid for three months.