Cell Free Protein Expression with HiBiT Quantification

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Expression with HiBiT Quantification is the fastest path from a sequence to protein yield metrics. Using a proprietary reconstituted E. coli transcription-translation system, react ions are completed in 4–16 hours and can yield up to 3 mg/mL of target protein. To accurately quantify the expressed proteins directly from the crude reaction mixture without purification, this service utilizes the Promega Nano-Glo® HiBiT Lytic Detection System. This tier is designed for early-stage screening, novel construct evaluation, and rapid, data-driven triage of candidate sequences before committing resources to downstream optimization or purification. Show more