Description Our bioactive FGF-2 (FGF-basic) Bovine, E. coli Recombinant Protein (147 aa), also known as truncated Fibroblast Growth Factor 2, is a single, non-glycosylated, polypeptide chain that contains 147 amino acids and has a molecular mass of approximately 16.5 kDa. The FGF-2 is purified by proprietary chromatographic techniques. Summary FGF-basic, a member of the fibroblast growth factor (FGF) family, is a non-glycosylated, heparin-binding growth factor and signaling protein encoded by the FGF2 gene. It binds to and exerts effects via specific fibroblast growth factor receptor (FGFR) proteins. Like other FGF family members, FGF-basic possesses broad mitogenic and cell survival activities, and is involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. Additionally, FGF-basic is a critical component of human embryonic stem cell (hESCs) culture medium; the growth factor is necessary for the cells to remain in an undifferentiated state. As a result, FGF-basic has been used to develop chemically-defined, serum-free and feeder-free hESCs culture mediums. CAS Registry No. 106096-93-9 Other Names Truncated bovine Fibroblast Growth Factor-2, Cow FGF2, FGF-2, FGF basic, FGF-b, Basic fibroblast growth factor, bFGF, Heparin-binding growth factor 2, HBGF-2. Amino Acid Sequence MPALPEDGGS GAFPPGHFKD PKRLYCKNGG FFLRIHPDGR VDGVREKSDP HIKLQLQAEE RGVVSIKGVC ANRYLAMKED GRLLASKCVT DECFFFERLE SNNYNTYRSR KYSSWYVALK RTGQYKLGPK TGPGQKAILF LPMSAKS. Source E. coli Formulation Sterile filtered white lyophilized (freeze-dried) powder. The protein was lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4. Purity Purity greater than 97.0% as determined by: Analysis by RP-HPLC. Analysis by SDS-PAGE. Biological Activity The ED50 as determined by a cell proliferation assay using murine balb/c 3T3 cells is < 0.1 ng/ml, corresponding to a specific activity of > 1.0 ×107IU/mg. Reconstitution It is recommended to reconstitute the lyophilized FGF-basic in sterile PBS not less than 100 µg/ml, which can then be further diluted to other aqueous solutions. Shipping At ambient temperature. Upon receipt, store the product at the temperature recommended below. Storage/Expiration Lyophilized FGF-basic, although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution, FGF-basic should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles. Usage This product is intended for Laboratory Research Use Only. Not for use in diagnostic or therapeutic procedures. This product may not be used as a pharmaceutical or veterinary drug, agricultural product, or food additive. Resources Product Datasheet (PDS) Related Products ISOkine® FGF-Basic (FGF-2, bFGF) Human, Animal-Free Recombinant Protein FGF-2 (FGF-basic) Human, E. coli Recombinant Protein (154 aa) FGF-2 (FGF-basic) Human, E. coli Recombinant Protein (147 aa) FGF-2 (FGF-basic) Human, Plant Recombinant Protein (146 aa) FGF-2 (FGF-basic) Bovine, E. coli Recombinant Protein (155 aa) FGF-2 (FGF-basic) Mouse, E. coli Recombinant Protein (146 aa) FGF-2 (FGF-basic) Rat, E. coli Recombinant Protein (146 aa) Cytokines & Growth Factors
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