Description Our bioactive FGF-2 (FGF-basic) Bovine, E. coli Recombinant Protein (155 aa), also known as Fibroblast Growth Factor 2, is a single, non-glycosylated, polypeptide chain that contains 155 amino acids and has a 17250 Dalton molecular mass. The FGF-2 is purified by proprietary chromatographic techniques. Summary FGF-basic, a member of the fibroblast growth factor (FGF) family, is a non-glycosylated, heparin-binding growth factor and signaling protein encoded by the FGF2 gene. It binds to and exerts effects via specific fibroblast growth factor receptor (FGFR) proteins. Like other FGF family members, FGF-basic possesses broad mitogenic and cell survival activities, and is involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. Additionally, FGF-basic is a critical component of human embryonic stem cell (hESCs) culture medium; the growth factor is necessary for the cells to remain in an undifferentiated state. As a result, FGF-basic has been used to develop chemically-defined, serum-free and feeder-free hESCs culture mediums. CAS Registry No. 106096-93-9 Other Names Bovine Fibroblast Growth Factor-2, Cow FGF2, FGF-2, FGF basic, FGF-b, Basic fibroblast growth factor, bFGF, Heparin-binding growth factor 2, HBGF-2. Amino Acid Sequence The sequence of the first five N-terminal amino acids was determined and was found to be Met-Ala-Ala-Gly-Ser. Source E. coli Formulation Sterile filtered white lyophilized (freeze-dried) powder. The protein was lyophilized from a concentrated (1 mg/ml) sterile solution containing 1% HSA. Purity Purity greater than 97.0% as determined by: Analysis by RP-HPLC. Analysis by SDS-PAGE. Biological Activity The ED50, measured in a mitogenic assay using quiescent NR6R-3T3 fibroblasts was found to be
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