GST-tag Elution Buffer Catalog number: B2016310 Lot number: Batch Dependent Expiration Date: Batch dependent Amount: 50 mL Molecular Weight or Concentration: N/A Supplied as: Powder Applications: a molecular tool for various biochemical applications Storage: −20°C Keywords: GSH Grade: Biotechnology grade. All products are highly pure. All solutions are made with Type I ultrapure water (resistivity >18 MΩ-cm) and are filtered through 0.22 um. References Schäfer F, Seip N, Maertens B, Block H, Kubicek J. Purification of GST-Tagged Proteins Methods Enzymol. 2015;559:127-39. Luo L, King NP, Yeo JC, Jones A, Stow JL. Single-step protease cleavage elution for identification of protein-protein interactions from GST pull-down and mass spectrometry Proteomics. 2014 Jan;14(1):19-23. Greenfield EA, DeCaprio J, Brahmandam M. Preparing GST-, His-, or MBP-Fusion Proteins from Bacteria Cold Spring Harb Protoc. 2020 Sep 1;2020(9):100024. Harper S, Speicher DW. Purification of proteins fused to glutathione S-transferase Methods Mol Biol. 2011;681:259-80. Deceglie S, Lionetti C, Roberti M, Cantatore P, Polosa PL. Expression and purification of large active GST fusion enzymes Methods Mol Biol. 2014;1129:169-80. Zwicker N, Adelhelm K, Thiericke R, Grabley S, Hänel F. Strep-tag II for one-step affinity purification of active bHLHzip domain of human c-Myc Biotechniques. 1999 Aug;27(2):368-75. Di Tomasso G, Dagenais P, Desjardins A, Rompré-Brodeur A, Delfosse V, Legault P. Affinity purification of RNA using an ARiBo tag Methods Mol Biol. 2012;941:137-55. Lichty JJ, Malecki JL, Agnew HD, Michelson-Horowitz DJ, Tan S. Comparison of affinity tags for protein purification Protein Expr Purif. 2005 May;41(1):98-105. Czepczyńska-Krężel H, Czerwinski M, Krężel A, Krop-Watorek A. Isolation of carcinoembryonic antigen N-terminal domains (N-A1) from soluble aggregates Protein Expr Purif. 2011 Jul;78(1):78-85.
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