His-tag Elution Buffer Catalog number: B2016356 Lot number: Batch Dependent Expiration Date: Batch dependent Amount: 50 mL Molecular Weight or Concentration: N/A Supplied as: Solution Applications: a molecular tool for various biochemical applications Storage: RT Keywords: His Elution Buffer Grade: Biotechnology grade. All products are highly pure. All solutions are made with Type I ultrapure water (resistivity >18 MΩ-cm) and are filtered through 0.22 um. References Sensi S, Goebel A. Human Auto-IgG Purification from High Volume Serum Sample by Protein G Affinity Purification Bio Protoc. 2022 Dec 5;12(23):e4562. Kish WS, Roach MK, Sachi H, Naik AD, Menegatti S, Carbonell RG. Purification of human erythropoietin by affinity chromatography using cyclic peptide ligands J Chromatogr B Analyt Technol Biomed Life Sci. 2018 May 15;1085:1-12. Kenna JG, Major GN, Williams RS. Methods for reducing non-specific antibody binding in enzyme-linked immunosorbent assays J Immunol Methods. 1985 Dec 27;85(2):409-19. Mechetner L, Sood R, Nguyen V, Gagnon P, Parseghian MH. The effects of hitchhiker antigens co-eluting with affinity-purified research antibodies J Chromatogr B Analyt Technol Biomed Life Sci. 2011 Sep 1;879(25):2583-94. Zhao JC, Zhao ZD, Wang W, Gao XM. Prokaryotic expression, refolding, and purification of fragment 450-650 of the spike protein of SARS-coronavirus Protein Expr Purif. 2005 Feb;39(2):169-74. Volpe G, Sozzo U, Piermarini S, Delibato E, Palleschi G, Moscone D. Towards the development of a single-step immunosensor based on an electrochemical screen-printed electrode strip coupled with immunomagnetic beads Anal Bioanal Chem. 2013 Jan;405(2-3):655-63. Swartz AR, Xu X, Traylor SJ, Li ZJ, Chen W. One-step affinity capture and precipitation for improved purification of an industrial monoclonal antibody using Z-ELP functionalized nanocages Biotechnol Bioeng. 2018 Feb;115(2):423-432. Husby S, Holmskov-Nielsen U, Jensenius JC, Erb K. Increased non-specific binding of heat-treated proteins to plastic surfaces analyzed by ELISA and HPLC-fractionation J Immunoassay. 1985;6(1-2):95-110. Kiess W, Kessler U, Funk B, Hoeflich A. Perturbation of C6 glial cells by acetate leads to modulation of [125I]IGF-II binding to the IGF-II/M6P receptor: implications for ligand internalization studies Biochim Biophys Acta. 1994 Sep 8;1223(2):179-84.
