Calmodulin-tag Elution Buffer Catalog number: B2016406 Lot number: Batch Dependent Expiration Date: Batch dependent Amount: 50 mL Molecular Weight or Concentration: N/A Supplied as: Solution Applications: a molecular tool for various biochemical applications Storage: RT Keywords: Calmodulin Elution Buffer Grade: Biotechnology grade. All products are highly pure. All solutions are made with Type I ultrapure water (resistivity >18 MΩ-cm) and are filtered through 0.22 um. References Gerace E, Moazed D. Affinity Purification of Protein Complexes Using TAP Tags Methods Enzymol. 2015;559:37-52. Zheng CF, Simcox T, Xu L, Vaillancourt P. A new expression vector for high level protein production, one step purification and direct isotopic labeling of calmodulin-binding peptide fusion proteins Gene. 1997 Feb 20;186(1):55-60. Völkel P, Le Faou P, Angrand PO. Interaction proteomics: characterization of protein complexes using tandem affinity purification-mass spectrometry Biochem Soc Trans. 2010 Aug;38(4):883-7. Gerace E, Moazed D. Affinity Purification of Protein Complexes Using TAP Tags Methods Enzymol. 2015;559:37-52. Zilio N, Boddy MN. Improved Tandem Affinity Purification Tag and Methods for Isolation of Proteins and Protein Complexes from Schizosaccharomyces pombe Cold Spring Harb Protoc. 2017 Mar 1;2017(3):pdb.prot091611. Desai UA, Sur G, Daunert S, Babbitt R, Li Q. Expression and affinity purification of recombinant proteins from plants Protein Expr Purif. 2002 Jun;25(1):195-202. Shanmugabalaji V, Douet V, Agne B, Kessler F. Affinity Purification of Chloroplast Translocon Protein Complexes Using the TAP Tag J Vis Exp. 2018 Nov 1;(141). Blackwell C, Brown JD. The application of tandem-affinity purification to Candida albicans Methods Mol Biol. 2009;499:133-48. Egorov MV, Tigerström A, Pestov NB, Korneenko TV, Kostina MB, Shakhparonov MI, Rydström J. Purification of a recombinant membrane protein tagged with a calmodulin-binding domain: properties of chimeras of the Escherichia coli nicotinamide nucleotide transhydrogenase and the C-terminus of human plasma membrane Ca2+ -ATPase Protein Expr Purif. 2004 Jul;36(1):31-9.
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