PRODUCT SPECIFICATION ITEM# 4006: Recombinant HCV NS5b Protein SPECIFICATIONS Concentration: see vial Mass/vial: see vial Volume/vial: see vial Diluent: PBS, 10% Glycerol Purity >95% Stabilizer: None Preservative: None Storage: -75o C Stability: At least 24 months at -75o C DESCRIPTION: HCV NS5b DNA sequence expressed as a fusion protein in the E. coli expression system. MW: approx. 78kD APPLICATIONS: In -vitro HCV Diagnostics,Western Analysis. PURIFICATION: HCV NS5b protein was purified by solvent extraction and preparative gel electrophoresis to >95% purity as determined by SDS-PAGE, reduced. SPECIFICITY: This protein binds to murine monoclonal antibodies specific for HCV NS5 protein. NS5b protein shows strong binding to HCV converted human serum polyclonal antibodies. BIOLOGICAL ACTIVITY: Not determined. APPLICATION AND INSTRUCTIONS FOR USE Recommended concentrations for use are approximate values. Dilute the antigen preparation at least 10 to 20-fold in PBS for solid phase antigen-antibody titration assays. A dose dependent response assay should be performed to determine the optimal concentration for use in specific applications.
