PRODUCT SPECIFICATIONS ITEM# 4004: Recombinant HCV NS3 Protein (E coli) SPECIFICATIONS Concentration: see vial Mass/vial: see vial Diluent: PBS, pH 7.4, 0.05% Sarcosyl. Purity: >95% Stabilizer: None Preservative: None Storage: -75o C Stability: At least 12 months at -75o C DESCRIPTION: Hepatitis C recombinant NS3 protein expressed as a fusion protein produced in the E.coli expression system. Molecular Size: approx: 101kD. APPLICATIONS: In vitro-HCV Diagnostics, T-cell Activation, Elisa western. PURIFICATION: HCV rNS3 is purified by ion-affinity chromatography and preparative electrophoresis methods to >95% purity as determined by SDS-PAGE, reduced. SPECIFICITY: This protein binds to murine monoclonal antibodies and shows strong reactivity with HCV converted human serum polyclonal antibodies in ELISA and Western ELISA. BIOLOGICAL ACTIVITY: Not determined. APPLICATION AND INSTRUCTIONS FOR USE Recommended concentrations for use are approximate values. Dilute the antigen preparation at least 10 to 20-fold in appropriate buffers for solid phase antigen-antibody titration assays. A dose dependent response assay should be performed to determine the optimal concentration for use in specific applications. HCV NS3 protein shows relatively strong binding specificity for HCV-specific human antibodies. ELISA and Western ELISA require protein in 10ng range depending on the nature and affinity of the secondary detection reagent. HCV converted human serum polyclonal antibodies yield titers of 1:10,000 or greater at 100ng of immobilized protein under standard ELISA conditions.
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