Human Hemoglobin for Interference Testing Catalog # K2010001D Lot # Batch Dependent Expiration Batch Dependent Volume 1 mL Appearance Dark red-brown Concentration 200 mg/mL Matrix Filtered solution Storage –20°C or lower; aliquot to avoid freeze-thaw Other Names Hb, human Hb, human haemoglobin This high-concentration human hemoglobin solution (200 mg/mL) is formulated for interference testing in clinical and biochemical assay workflows. Supplied as a dark red-brown filtered solution, it enables researchers to simulate hemolysis-related interference and assess assay robustness under physiologically relevant conditions. The product is packaged in 1 mL aliquots and should be stored at –20°C or lower to preserve integrity. Hemoglobin is a known interferent in immunoassays, spectrophotometric platforms, and enzymatic reactions. This reagent allows for controlled spiking into specimens to evaluate signal distortion, cross-reactivity, and matrix effects. Applications include method validation, QC benchmarking, and interference panel construction. The solution is filtered for clarity and consistency, and should be aliquoted upon receipt to avoid repeated freeze-thaw cycles. Key benefits: High-purity human hemoglobin solution for interference testing 200 mg/mL concentration enables robust spiking protocols Filtered for clarity and reproducibility Supports immunoassay, spectrophotometry, and enzymatic platforms Stable at –20°C; aliquot recommended Indication for Use:This product is for Research Use Only (RUO). This product is not for administration to humans or animals. This product is not for human or veterinary diagnostic or therapeutic use. All Interference Products Brochure Download PDF References: Schiettecatte J et al. Interferences in Immunoassays. Adv Immunoassay Tech. 2012. Miller JJ, Levinson SS. Interferences In Immunoassays. Immunoassay. 1996;165–190. Ward G. Immunoassay interference investigations. Pathology. 2013;45. Park JY, Kricka LJ. Immunoassay interference. Immunoassay Handbook. 2013;403–416. EP07: Interference Testing in Clinical Chemistry. CLSI. 2020. Gilles A et al. Targeting the 80S ribosome in cancer. Cells. 2020;9(3):E629. Coussens NP et al. Assay interference in proximity assays. Assay Guidance Manual. 2020. Wang L et al. Fluorescent probes for nitric oxide. Org Biomol Chem. 2020;18(8):1522–1549. Kawasaki H et al. Drosophila GBA mutant phenotypes. Gene. 2017;614:49–55. Hull B. HAMA interference with TSH assay. J S C Med Assoc. 2012;108(1):12–13. Degn SE et al. Rat kappa light-chain interference. J Immunol Methods. 2011;372(1–2):204–8.
