Description Hieff Trans Liposomal Transfection Reagent is a versatile liposome transfection reagent, suitable for DNA, RNA, and oligonucleotide transfection, with high transfection efficiency for most eukaryotic cells. Its unique formula allows it to be added directly to the medium, and the presence of serum does not affect transfection efficiency, which reduces the damage to cells caused by serum removal. There is no need to remove the nucleic acid-Hieff Trans™ complex or replace it with a fresh medium after transfection, and it can also be removed after 4-6 hours. Hieff Trans is supplied in sterile liquid form. Usually, for 24-well plate transfection, about 1.5 μL each time, 1 mL of Hieff Trans™ can do about 660 transfections; for a 6-well plate, about 6 μL each time, 1 mL of Hieff Trans™ can do about 660 transfections. 160 transfections. Feature Exceptional Efficiency: Superior transfection performance in the broad range of cell lines, including transient transfection and stable transfection Wide Adaptability: Excellent transfection efficiency in a variety of cell lines and high levels of recombinant protein expression Low Toxicity: The activities of the diverse cells almost unaffected by transfection reagents from YEASEN Simple Operation: Proven efficacy in the presence of serum—eliminates the need to change media following transfection Cost Effective: Competitive transfection effect with more affordable prices Application Cell Transfection Specification Form Liquid Serum Compatible Yes Cell Type Established Cell Lines Sample Type Plasmid DNA, Synthetic siRNA Transfection Technique Lipid-Based Transfection Components Components No. Name 40802ES02 40802ES03 40802ES08 40802 Hieff Trans Liposomal Transfection Reagent 0.5 mL 1 mL 5×1 mL Shipping and Storage The product is shipped with ice packs and can be stored at 2-8ºC for one year. Do not freeze! Figures Demonstration of Transfection Effect Figure 1. Hieff Trans™ Liposome Transfection Reagent outperforms the transfection reagent from the competitive brand（T* brand in Figure 1a, P* brand in Figure 1b). Each reagent was used to transfect the target cell line（Hela cell line in Figure 1a, DF-1 cell line in Figure 1b）in a 96-well format. GFP expression was analyzed 48 hours posttransfection. Hieff Trans™ Liposome Transfection Reagent provided higher GFP transfection efficiency than the competitive brand products. Validated Cell Lines Product Name Hieff TransTMLiposomal Transfection Reagent Validated Cell Lines 293T Calu 1 HEK293 HO1980 N2A TS 293F CHO-K1 HEK293T HUVEC NCI-H1975 U-87 293FT COS-7 Hela MCF10A NIH-3T3 Vero 3T3 DF-1 Hep2C MCF-7 Neuro-2a WEHI A549 H1299 Hep3B MDA-MB-231 PC-12 WRL-68 BV-2 H520 Hepa1-6 MDA-MB-231-LM2-4175 Raw264.7 B50 HaCaT HepG2 MDCK SGC-7901 C2C12 HCT116 HK2 MEF T47D … Figure 2. List of cell lines successfully transfected with Hieff Trans™ Liposomal Transfection Reagent (Under continuous update). Documents: CoA 40802-H2121061 CoA.pdf Safety Data Sheet 40802-Hieff Trans™ Liposomal Transfection Reagent-MSDS-HB220419.pdf Manuals 40802_Manual_HB221130_EN.pdf
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