PRODUCT SPECIFICATION Product# 1101: Murine anti-gp120 IIIB Monoclonal Antibody SPECIFICATIONS APPLICATIONS Antibody Class: IgG1 ELISA, Western ELISA Concentration: See vial Immunoprecipitation Mass/vial: 100µg Immunocytochemistry Purity: >95% Immunohistochemistry Stabilizer: None In Vitro Virus Neutralization Preservative: None Physical State: Frozen Liquid Storage: -75oC Stability: At least 12 months at -75oC DESCRIPTION: High affinity murine anti-gp120 IIIB mAb. SPECIFICITY: Binds to V3-loop of HIV-1 IIIB gp120/gp160. This mAb shows 5-10% cross reactivity with other HIV-1 strains. BIOLOGICAL ACTIVITY: Neutralizes HIV-1 IIIB at 1-10µg/ml as determined by syncycium assays with CD4-bearing HIV-1 IIIB infected cells and co-cultures of infected PBLs. APPLICATIONS AND INSTRUCTIONS FOR USE Recommended dilutions for use are approximate values. A dose dependent response assay should be performed to determine the optimal concentration for use in specific applications. ELISA may be performed at 10-100ng/ml of mAb depending on the amount of immobilized antigen in the assays. Western ELISA requires 1-5µg of mAb/ml. RELATED PRODUCTS PRODUCT#: 1101-P Peroxidase-Conjugated Murine anti-gp120 HIV-1 IIIB Application: ELISA, Western ELISA, Histocytochemistry, Histocytology. PRODUCT#: 1101-F Fluorescene Murine anti-gp120 HIV-1 IIIB Application: FACS, Cell Staining PRODUCT#: 1101-G Colloidal Gold-Conjugated Murine anti-gp120 HIV-1 IIIB Application: Dot Blots, EM.
