Description This product is derived from recombinant E. coli carrying Bacillus subtilis phage phi29 clone gene 2, which was expressed by E. coli and isolated and purified multiple times. Phi29 is a DNA polymerase with high sequential synthesis capacity and strand displacement for efficient isothermal DNA amplification with 3'–5' exonuclease (proofreading) activity. The catalog number has been updated from P310141-0001 to P310141-1KU. Specification Full name Phi29 DNA Polymerase Composition Phi29 DNA Polymerase (10 U/μL), 10 × Phi29 Buffer. Reaction Buffer 10X phi29 Buffer Unit Definition 1 U enzyme is the enzyme required to catalyze 0.5 pmol dCMP into a polynucleotide fragment within 10 min at 30°C. Thermal Inactivation Heat at 60°C for 10 min to lose activity. Concentration 10 U/μL Storage Buffer 50 mM Tris-HCl (pH 7.5), 0.1 mM EDTA, 1 mM DTT, 100 mM KCl, 0.5% (v/v) Nonidet P40, 0.5% (v/v) Tween 20, 50% (v/v) glycerol. Component Phi29 DNA Polymerase (10 U/μL), 10 × Phi29 Buffer. Features High-precision DNA synthesis Extremely high amplified DNA yield can be obtained even from a very small amount of template The amplification products can be directly used in downstream applications (PCR, restriction enzyme digestion, SNP genotyping, etc.). Applications Routine PCR High throughput PCR Multiplex PCR Genotyping Rolling circle amplification (RCA) Storage -20°C
