Overview TriAltus’ Phi29 DNA polymerase is a purified enzyme commonly used in isothermal DNA amplification workflows, including whole genome amplification and rolling circle amplification. Known for its strand-displacement activity and high processivity, Phi29 DNA polymerase supports applications requiring robust DNA synthesis under defined reaction conditions. Also known as: Phi29 DNA polymerase enzyme, bacteriophage Phi29 DNA polymerase, φ29 DNA polymerase, Phi29 polymerase Why Performance and Consistency Matter for Phi29 DNA Polymerase For DNA amplification workflows, enzyme purity and lot-to-lot consistency directly influence reaction reliability and experimental reproducibility. Impurities or variability in polymerase preparations can affect amplification efficiency, background signal, and downstream analysis. High-purity, well-characterized Phi29 DNA polymerase supports more predictable amplification behavior across experiments, particularly in workflows where consistent DNA synthesis is critical. Bulk Supply & Program Support TriAltus supports bulk supply and tailored formats for organizations requiring dependable, long-term access to critical enzymes. Production under ISO 13485–compliant processes and the CLīM™ purification platform enable batch consistency and efficient cost-of-goods at scale, supporting volume-based pricing for sustained programs. Contact TriAltus to discuss volume, documentation, packaging, and supply planning. Key Benefits Highly processive, isothermal enzyme Exceptional strand displacement Ability to synthesize long DNA fragments up to 70 kb 3’→5’ proofreading exonuclease supporting high-fidelity amplification Specifications PURITY | ≥95% ENZYME SOURCE | Phi29 DNA polymerase (from Bacillus subtilis phage phi29) is expressed in E. coli and is purified with our CLīM technology. STORAGE/SHIPPING CONCENTRATION | 10 U/µL STORAGE BUFFER | 10 mM Tris-Cl pH 7.4, 100 mM KCl, 0.1 mM EDTA, 1 mM DTT, 0.5% Tween® 20, 0.5% IGEPAL® CA-630, 50% glycerol RECOMMENDED STORAGE CONDITIONS | -20°C EXPIRATION | 6 months from receipt when stored as directed. Avoid repeated freeze/thaw cycles. Produced under ISO 13485–compliant processes. Phi29 DNA Polymerase Data Sheet One unit is defined as the amount of enzyme required to convert 0.5 pmol dNTPs to acid-insoluble material in 10 min at 30°C. SKU List 3,000 Units: 40-2414 | 10,000 Units: 40-2420 | Bulk: Inquire Applications Whole Genome Amplification (WGA) Phi29 DNA polymerase is commonly used in WGA workflows to amplify limited DNA samples under isothermal conditions for downstream analysis. Rolling Circle Amplification (RCA) The strand-displacing activity of Phi29 supports RCA applications for generating long DNA products from circular templates. Method Development and Optimization Purified Phi29 DNA polymerase is suitable for protocol development and optimization where consistent enzyme performance supports reproducible results. Learn More Learn how TriAltus achieves high purity using CLīM™ purification. Explore peer-reviewed publications describing the underlying protein production platform. View the Purified CRISPR-Cas9 Protein page for comparison with other molecular biology enzymes. FAQs Does Phi29 DNA polymerase have strand displacement activity? Yes. Phi29 DNA polymerase exhibits strong strand displacement activity, which enables continuous DNA synthesis without thermal cycling and supports isothermal amplification workflows such as RCA and MDA. Does Phi29 DNA polymerase have proofreading activity? Yes. Phi29 DNA polymerase has 3’→5’ exonuclease proofreading activity, which contributes to high-fidelity DNA synthesis in amplification workflows. What types of DNA templates can Phi29 DNA polymerase amplify? Phi29 DNA polymerase can amplify linear and circular DNA templates and is commonly used with genomic DNA, plasmid DNA, and circular DNA substrates in isothermal amplification workflows. Is Phi29 DNA polymerase suitable for low-input or single-cell applications? Phi29 DNA polymerase is frequently used in low-input DNA amplification workflows, including applications such as single-cell genomics and metagenomics, where efficient amplification from limited starting material is required.
