Product Specifications: Item# 30180: Recombinant Dengue Antigen D3 Envelope Protein (Baculo) Concentration: See vial Mass/vial: See vial Diluent: 5mM Hepes, pH8.8, 7.5mM NaCI 0.5% Glycerol, 0.02M Beta ME 0.01% Triton XIOO Purity: >95% Stabilizer: None Preservative: None Storage: -750C, 20°C Physical State: Liquid Stability: At least 36 months at -75°C. Applications: Elisa/Western Elisa, Diagnostics Description: Synthetic Dengue virus D3 Envelope DNA sequence, expressed in the Baculo Expression System. Purification: This protein is purified by Ni affinity chromatography to >95% purity as determined by SDS-PAGE, reduced. Molecular Weight: approx. 42.9kD. Specificity: This protein binds to Dengue virus converted human sera as determined by Elisa, Western Blots and by one step lateral flow diagnostic testing. Application and Instructions for use Recommended concentrations for use are approximate values. Elisa is performed in 1-2ug/ml protein range for tittering Dengue virus converted human polyclonal antibodies. Concentrations if 1-2mg/ml (1-2ul/cm) are recommended for Lateral Flow Diagnostics.
