Description Super Pfx DNA Polymerase is a fast, high-efficiency, high-fidelity DNA polymerase with 5'-3' DNA polymerase activity and 3'-5' exonuclease activity. This polymerase is modified from other high-fidelity enzymes, has strong amplification ability, rapid amplification speed (4-6 kb/min), and high fidelity. This polymerase overcomes some defects of Pfu polymerase such as the poor amplification ability, low yield and amplification rate, which greatly shortens the reaction time. This product can be used for the amplification of long fragments and other various complex templates. The PCR product does not have an "A" base at the 3' end and can be directly used for blunt-end cloning. For T/A cloning, it is necessary to add "A" to the end of the PCR product. This product has the characteristics of rapid extension, high amplification efficiency and high fidelity. It is suitable for experiments such as gene cloning, site-directed mutagenesis, and SNP amplification. Application Conventional PCR reactions, gene cloning, site-directed mutagenesis, and SNP amplification. Components Components CW2848S CW2848M Super Pfx DNA Polymerase, 2U/μL 50 μL 250 μL 2×Super Pfx Buffer 2×1.25 mL 7×1.8 mL dNTP Mix，10mM each 150 μL 750 μL Shipping and Storage This product is shipped with ice packs and stored at -15℃ ~ -25℃. Documents: Manuals Download here
