Universal Protease Substrate Catalog number: B2013021 Lot number: Batch Dependent Expiration Date: Batch dependent Amount: 10 mg Molecular Weight or Concentration: 1 mg/mL Supplied as: Lyophilized Powder Applications: molecular tool for various biochemical applications Storage: -20°C Keywords: Substrate of protease, casein, resorufin-labeled, protease substrate, universal Grade: Biotechnology grade. All products are highly pure. All solutions are made with Type I ultrapure water (resistivity >18 MΩ-cm) and are filtered through 0.22 um. References Vuorinen E, Valtonen S, Hassan N, Mahran R, Habib H, Malakoutikhah M, Kopra K, Härmä H. Protease Substrate-Independent Universal Assay for Monitoring Digestion of Native Unmodified Proteins Int J Mol Sci. 2021 Jun 14;22(12):6362. Ponomariov M, Shabat D, Green O. Universal Access to Protease Chemiluminescent Probes through Solid-Phase Synthesis Bioconjug Chem. 2021 Oct 20;32(10):2134-2140. Yocum RR, Rasmussen JR, Strominger JL. The mechanism of action of penicillin. Penicillin acylates the active site of Bacillus stearothermophilus D-alanine carboxypeptidase J Biol Chem. 1980 May 10;255(9):3977-86. Dubiel A, Wegrzyn K, Kupinski AP, Konieczny I. ClpAP protease is a universal factor that activates the parDE toxin-antitoxin system from a broad host range RK2 plasmid Sci Rep. 2018 Oct 16;8(1):15287. Li S, Hsieh KY, Kuo CI, Su SC, Huang KF, Zhang K, Chang CI. Processive cleavage of substrate at individual proteolytic active sites of the Lon protease complex Sci Adv. 2021 Nov 12;7(46):eabj9537. Zhao LY, Sun YL, Mao L, Lian HZ, Sheng D. Universal gold nanoparticle modified hybrid monolithic substrate developed for facile in-column post-functionalization Talanta. 2021 Apr 1;225:121993. Majumder P, Baumeister W. Proteasomes: unfoldase-assisted protein degradation machines Biol Chem. 2019 Dec 18;401(1):183-199. Kim HT, Goldberg AL. S5a/Rpn10, a UIM-protein, as a universal substrate for ubiquitination Methods Mol Biol. 2012;832:653-60. Kudriaeva A, Kuzina ES, Zubenko O, Smirnov IV, Belogurov A Jr. Charge-mediated proteasome targeting FASEB J. 2019 Jun;33(6):6852-6866.
