The RT-LAMP Probe Assay Kit is a reagent designed for LAMP amplification using DNA and RNA templates. It includes the next-generation Bst Plus HSS DNA Polymerase, heat-resistant Hifair™ III Reverse Transcriptase, MRI, and an optimized buffering system, allowing for rapid and efficient LAMP/RT-LAMP probe-based detection. The Bst Plus HSS DNA Polymerase employs an aptamer-based hot start mechanism that suppresses enzymatic activity at ambient temperatures, enhancing specificity and sensitivity while enabling stable reaction assembly at room temperature. Features Hotstart Bst DNA Pol High Sensitivity Components Components No. Name 16903ES60(100 T) 16903-A 4×RT-LAMP Buffer (HSS) 600 µL 16903-B RT-LAMP Enzyme mix (HSS) 100 µL Specifications Detection method RT-LAMP Fluorescent probe method Enzyme mix Bst Plus HSS DNA Polymerase; Hifair™ III Reverse Transcriptase Type of sample RNA Application Isothermal amplification reaction Shipping and Storage The product should be stored at -15℃ ~ -25℃ for 1 year. Please avoid light. Operating Instructions Components Volume (μL) Final Concentration 4×RT-LAMP Buffer(HSS) 6 1 × RT-LAMP Enzyme mix(HSS) 1 - Primer mix* 2.5 - Template 1-10 - ddH2O up to 25 - Notes: Recommended primer set at 16 μM FIP/BIP, 2 μM F3/B3, and 4 μM LoopF/LoopB; the probe can be added at the optimal amount confirmed by the design scheme, and the ratio of other optimal primer sets can be tested. Reaction conditions Incubate at a constant temperature of 63-65°C for 30-60 minutes, followed by deactivation at 85°C for 5 minutes. Notes For your safety and health,please wear lab coats and disposable gloves for operation.
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