The RT-LAMP Fluorescent Assay Kit (Bst Plus) is a kit capable of isothermal amplification using DNA and RNA as templates. This kit employs the next-generation aptamer-encapsulated Bst Plus DNA Polymerase and heat-resistant HifairTM III Reverse Transcriptase, along with an MRI, combined with a finely optimized buffer system, to achieve highly efficient, rapid, and highly specific detection reactions, and supports room-temperature reaction system preparation. Features Broad Compatibility: Compatible with over 20 animal and human pathogen targets, with GC content ranging from 30% to 65%. Delivers comparable amplification times to leading products. High Specificity: Validated across diverse DNA and RNA targets. Demonstrates superior specificity with no false positives within 60 minutes and delayed amplification in NTCs compared to competitors. Excellent Sensitivity: Capable of detecting African swine fever virus at 15 copies/reaction and Influenza B virus at 10 copies/reaction. Robust Stability: Maintains performance after 20 freeze-thaw cycles and 7 days at 37°C. Supports convenient room temperature reaction setup. Components Components No. Name 16729ES60 16729ES80 16729-A 4×LAMP Buffer 600 µL 6 mL 16729-B Bst Plus DNA Polymerase 100 µL 1 mL 16729-C RT Enzyme mix 50 µL 500 µL 16729-D 25×Fluorescent Dye 100 µL 1 mL Shipping and Storage This product should be stored at -25~-15℃ for 1 year.Component D must be stored away from light. Application Isothermal Amplification;Rapid detection of pathogenic bacteria. Figures 1. Workflow Figure 1. RT-LAMP Workflow. 2. Broad Compatibility: DNA Pathogen Detection&RNA Pathogen Detection Figure 2. Broad Pathogen Detection Compatibility. (A) DNA pathogen detection: Reliable amplification observed across multiple DNA virus targets. (B) RNA pathogen detection: Effective detection of RNA viruses with high sensitivity and specificity. 3. High Specificity Figure 3. High Specificity of RT-LAMP Assay (Cat#16729) Compared to Supplier A* Specificity was evaluated using both DNA and RNA templates, including positive targets and non-target (negative) controls. The RT-LAMP assay (Cat#16729) showed no false positives and clear separation between positive and negative samples, outperforming Supplier A* by avoiding non-specific amplification. 4. Strong stability Figure 4. Stability Evaluation of the RT-LAMP System. (A) Freeze-thaw stability: Performance remains consistent after 20 freeze-thaw cycles. (B) Thermal accelerated stability: Maintains amplification efficiency after 7 days of storage at 37°C. Documents: Safety Data Sheet 16729_MSDS_HB250713_EN.PDF Manuals 16729_Manual_Ver.EN20250708.pdf
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