Basic fibroblast growth factor (bFGF) is a member of the fibroblast growth factor (FGF) family, which is a growth factor and signaling protein encoded by the FGF2 gene. FGF family members have a wide range of activities that promote mitosis and cell survival, and are involved in various biological processes including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth, and invasion. In normal tissues, bFGF exists in the extracellular matrix beneath the basement membrane and vascular endothelium. bFGF can stimulate and regulate the differentiation and proliferation of various cells originating from the mesoderm and neuroectoderm, including vascular endothelial cells, epithelial cells, myoblasts, osteoblasts, and glial cells. FGF-2 plays an important role in embryonic development and tissue healing. bFGF is the fundamental component that maintains pluripotency in all embryonic and induced pluripotent stem cell culture media. The product is expressed in E. coli and provided in liquid form, with high activity, high purity and low endotoxin. Product Properties Synonyms bFGF, FGF basic, FGF2, FGF-2, fibroblast growth factor 2 (basic), HBGF-2, Prostatropin. Source Human bFGF Protein is expressed from E.coli without tag. Sequence Ala 144 - Ser 288. Molecular Weight Approximately 16.29 kDa. Accession P09038. Biological Activity Human bFGF GMP Protein stimulates proliferation of murine balb/c 3T3 cells. The specific activity of Human bFGF GMP Protein is >8.0 x 10^5 IU/mg, which is calibrated against Basic Fibroblast Growth Factor WHO International Standard (NIBSC code: 90/712). Purity ＞95% as determined by SDS-PAGE and HPLC. Endotoxin ＜0.01 EU/μg protein as determined by LAL method. Sterility Negative. Mycoplasma Negative. Vitro Virus Negative. Host Cell Protein Less than 0.5 ng/μg when tested by ELISA. Host Cell DNA Less than 20 ng/mg when tested by qPCR. Formulation Liquid from 0.22 μm filtered solution in PBS. Physical Appearance Liquid. Storage The product should be stored at -85~-65℃ for 2 years. Notes 1.Please operate with lab coats and disposable gloves, for your safety. 2.This product is for research use only. Assay Data SDS-PAGE Figure 1. The purity is greater than 95% as determined by SDS-PAGE under reduced condition. SEC-HPLC Figure 2. The purity is greater than 95% as determined by SEC-HPLC. Cell Based Assay Figure 3. Human bFGF GMP Protein stimulates proliferation of murine balb/c 3T3 cells. The specific activity of Human bFGF GMP Protein is >8.0 x 10^5 IU/mg, which is calibrated against Basic Fibroblast Growth Factor WHO International Standard (NIBSC code: 90/712). Documents: Manuals 91341-Manual-HB250721-EN.pdf
