Product Properties Alternative Names IL18R1,IL-1 R5,IL18Ra,CD218a Species Human Expression Sequence Ala19-Arg329 Accession AAH69575.1 Tag C-hFc Expression System HEK293 Molecular Weight The protein has a predicted MW of 62.52 kDa. Due to glycosylation, the protein migrates to 75-95 kDa based on SDS-PAGE result. Purity > 95% as determined by SDS-PAGE and HPLC. Endotoxin < 1.0 EU per μg by the LAL method. Formulation Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Appearance Lyophilized Powder Biological Activity 【SPR Data】Human IL-18 R1, hFc Tag captured on CM5 Chip via Protein A can bind Human IL-18, No Tag with an affinity constant of 0.73 nM as determined in SPR assay . Reconstitution Method Before opening, centrifuge briefly. Reconstitute the lyophilized protein with distilled water to a concentration exceeding 100 μg/mL. Aliquot after initial reconstitution to avoid repeated freeze-thaw cycles. Features High purity, high activity, low endotoxin, high batch-to-batch consistency Storage Lyophilized powder: Store at -25 to -15°C; stable for 1 year upon receipt.Reconstituted protein: Store under sterile conditions at -85 to -65°C; stable for 3 months.
