Calpain Fluorogenic Substrate Catalog number: B2011074 Lot number: Batch Dependent Expiration Date: Batch dependent Amount: 5 mg Molecular Weight or Concentration: 763.9 g/mol Supplied as: Lyophilized Applications: molecular tool for various chemical, biochemical and immunological applications Storage: -20 °C Keywords: Suc-LLVY-AMC Grade: Biotechnology grade. All products are highly pure. All solutions are made with Type I ultrapure water (resistivity >18 MΩ-cm) and are filtered through 0.22 um. References Campbell JS, Hallett MB. Active calpain in phagocytically competent human neutrophils: electroinjection of fluorogenic calpain substrate Biochem Biophys Res Commun. 2015 Feb 13;457(3):341-6. Cardoso M, Oliveira D, Araujo H. Expression and Activity of Calpain A in Drosophila melanogaster Methods Mol Biol. 2019;1915:93-101. Tompa P, Buzder-Lantos P, Tantos A, Farkas A, Szilágyi A, Bánóczi Z, Hudecz F, Friedrich P. On the sequential determinants of calpain cleavage J Biol Chem. 2004 May 14;279(20):20775-85. Mallya SK, Meyer S, Bozyczko-Coyne D, Siman R, Ator MA. A sensitive, continuously recording fluorogenic assay for calpain Biochem Biophys Res Commun. 1998 Jul 20;248(2):293-6. Koller G, Schürholz E, Ziebart T, Neff A, Frankenberger R, Bartsch JW. Clinical Evaluation of Pathognomonic Salivary Protease Fingerprinting for Oral Disease Diagnosis J Pers Med. 2021 Aug 30;11(9):866. Jennings S, Hallett MB. Single cell measurement of calpain activity in neutrophils reveals link to cytosolic Ca(2+) elevation and individual phagocytotic events Biochem Biophys Res Commun. 2019 Jul 12;515(1):163-168. Kitagawa Y, Tanaka S, Kamiya M, Kuriki Y, Yamamoto K, Shimizu T, Nejo T, Hana T, Matsuura R, Koike T, Yamazawa E, Kushihara Y, Takahashi S, Nomura M, Takami H, Takayanagi S, Mukasa A, Urano Y, Saito N. A Novel Topical Fluorescent Probe for Detection of Glioblastoma Clin Cancer Res. 2021 Jul 15;27(14):3936-3947. Ali MA, Stepanko A, Fan X, Holt A, Schulz R. Calpain inhibitors exhibit matrix metalloproteinase-2 inhibitory activity Biochem Biophys Res Commun. 2012 Jun 22;423(1):1-5. Sasaki T, Kikuchi T, Yumoto N, Yoshimura N, Murachi T. Comparative specificity and kinetic studies on porcine calpain I and calpain II with naturally occurring peptides and synthetic fluorogenic substrates J Biol Chem. 1984 Oct 25;259(20):12489-94.
