Enteropeptidase Fluorogenic Substrate Catalog number: B2012917 Lot number: Batch Dependent Expiration Date: Batch dependent Amount: 500 ug Molecular Weight or Concentration: 859.7 g/mol Supplied as: Lyophilized Powder Applications: molecular tool for various biochemical applications Storage: -20°C Keywords: (3S,6S,9S,12S,15S)-3-acetamido-19-amino-6,9,12-tris(carboxymethyl)-4,7,10,13-tetraoxo-15-((2-oxo-4-(trifluoromethyl)-2H-chromen-7-yl)carbamoyl)-5,8,11,14-tetraazanonadecanoic acid, trifluoroacetate salt Grade: Biotechnology grade. All products are highly pure. All solutions are made with Type I ultrapure water (resistivity >18 MΩ-cm) and are filtered through 0.22 um. References Yang X, Ding Z, Peng L, Song Q, Zhang D, Cui F, Xia C, Li K, Yin H, Li S, Li Z, Huang H. Cryo-EM structures reveal the activation and substrate recognition mechanism of human enteropeptidase Nat Commun. 2022 Nov 14;13(1):6955. Zheng XL, Kitamoto Y, Sadler JE. Enteropeptidase, a type II transmembrane serine protease Front Biosci (Elite Ed). 2009 Jun 1;1(1):242-9. Antonowicz I, Hesford FJ, Green JR, Grogg P, Hadorn B. The application of a new synthetic substrate to the determination of enteropeptidase in rat small intestine and human intestinal biopsies Clin Chim Acta. 1980 Feb 14;101(1):69-76. Azhar M, Somashekhar R. Production and purification of recombinant enteropeptidase expressed in an insect-baculovirus cell system Prep Biochem Biotechnol. 2015;45(3):268-78. Hesford F, Hadorn B, Blaser K, Schneider CH. A new substrate for enteropeptidase FEBS Lett. 1976 Dec 1;71(2):279-82. Choi MG, Lee E, Chung HS, Jang SH, Lee C. A fluorogenic method for measuring enteropeptidase activity: spectral shift in the emission of GD4K-conjugated 7-amino-4-methylcoumarin BMB Rep. 2011 Jul;44(7):458-61. Yang X, Yin H, Peng L, Zhang D, Li K, Cui F, Xia C, Huang H, Li Z. The Global Status and Trends of Enteropeptidase: A Bibliometric Study Front Med (Lausanne). 2022 Feb 10;9:779722. Smith ET, Johnson DA. Human enteropeptidase light chain: bioengineering of recombinants and kinetic investigations of structure and function Protein Sci. 2013 May;22(5):577-85. Ostapchenko VG, Gasparian ME, Kosinsky YA, Efremov RG, Dolgikh DA, Kirpichnikov MP. Dissecting structural basis of the unique substrate selectivity of human enteropeptidase catalytic subunit J Biomol Struct Dyn. 2012;30(1):62-73.
