Fluorogenic Elastase Substrate Catalog number: B2011110 Lot number: Batch Dependent Expiration Date: Batch dependent Amount: 1 mg Molecular Weight or Concentration: 1167 g/mol Supplied as: Lyophilized Applications: molecular tool for various biochemical applications Storage: -20 °C Keywords: Rhodamine Elastase Substrate Grade: Biotechnology grade. All products are highly pure. All solutions are made with Type I ultrapure water (resistivity >18 MΩ-cm) and are filtered through 0.22 um. References Al-Abdullah IH, Bagramyan K, Bilbao S, Qi M, Kalkum M. Fluorogenic Peptide Substrate for Quantification of Bacterial Enzyme Activities Sci Rep. 2017 Mar 13;7:44321. Rust L, Messing CR, Iglewski BH. Elastase assays Methods Enzymol. 1994;235:554-62. Sun Q, Li J, Liu WN, Dong QJ, Yang WC, Yang GF. Non-peptide-based fluorogenic small-molecule probe for elastase Anal Chem. 2013 Dec 3;85(23):11304-11. Lamort AS, Gravier R, Laffitte A, Juliano L, Zani ML, Moreau T. New insights into the substrate specificity of macrophage elastase MMP-12 Biol Chem. 2016 May;397(5):469-84. Johnson AF, Struthers MD, Pierson KB, Mangel WF, Smith LM. Nonisotopic DNA detection system employing elastase and fluorogenic rhodamine substrate Anal Chem. 1993 Sep 1;65(17):2352-9. Rudolphus A, Stolk J, van Twisk C, van Noorden CJ, Dijkman JH, Kramps JA. Detection of extracellular neutrophil elastase in hamster lungs after intratracheal instillation of E. coli lipopolysaccharide using a fluorogenic, elastase-specific, synthetic substrate Am J Pathol. 1992 Jul;141(1):153-60. Liu SY, Xiong H, Li RR, Yang WC, Yang GF. Activity-Based Near-Infrared Fluorogenic Probe for Enabling in Vitro and in Vivo Profiling of Neutrophil Elastase Anal Chem. 2019 Mar 19;91(6):3877-3884. Metzemaekers M, Cambier S, Blanter M, Vandooren J, de Carvalho AC, Malengier-Devlies B, Vanderbeke L, Jacobs C, Coenen S, Martens E, Pörtner N, Vanbrabant L, Van Mol P, Van Herck Y, Van Aerde N, Hermans G, Gunst J, Borin A, Toledo N Pereira B, Dos Sp Gomes AB, Primon Muraro S, Fabiano de Souza G, S Farias A, Proenca-Modena JL, R Vinolo MA; CONTAGIOUS Consortium, Marques PE, Wouters C, Wauters E, Struyf S, Matthys P, Opdenakker G, Marques RE, Wauters J, Gouwy M, Proost P. Kinetics of peripheral blood neutrophils in severe coronavirus disease 2019 Clin Transl Immunology. 2021 Apr 29;10(4):e1271. Anderson BM, Poole DP, Aurelio L, Ng GZ, Fleischmann M, Kasperkiewicz P, Morissette C, Drag M, van Driel IR, Schmidt BL, Vanner SJ, Bunnett NW, Edgington-Mitchell LE. Application of a chemical probe to detect neutrophil elastase activation during inflammatory bowel disease Sci Rep. 2019 Sep 16;9(1):13295.
