LAMP Probe Assay Kit (Hotstart Bst Plus, HSS) _ 16721ES

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The LAMP Probe Assay Kit (Bst Plus HSS) is a reagent designed for LAMP amplification using DNA as a template. It utilizes the next-generation Bst DNA Polymerase combined with an op timized buffer system to achieve highly sensitive amplification with excellent specificity. The buffer in this product does not contain nucleic acid dyes, making it suitable for probe-based detection. For fluorescence-based methods, additional dye needs to be added. Component Component Number Component Name 16721ES60 (100T) 16721-A 4×Bst Buffer 600 µL 16721-B Bst Plus HSS DNA Polymerase (40 U/µL) 100 µL Storage Conditions Store at -25°C to -15°C. Shelf life: 1 year. If not used for an extended period, store protected from light. Instructions for Use 1. Reaction System Component Volume (µL) Final Concentration 4×Bst Buffer 6 1× Bst Plus HSS DNA Polymerase (8U/µL) 1 - Primer mix* 2.5 - Template 1-10 - ddH2O up to 25 - *Primer mix: Recommended primer concentrations are 16 µM FIP/BIP, 2 µM F3/B3, and 4 µM LoopF/LoopB. The probe concentration should be optimized based on the design. Other primer set ratios may also be tested for optimal performance. 2. Reaction Conditions Incubate at 63-65°C for 30-60 minutes, followed by enzyme inactivation at 85°C for 5 minutes. Note For your safety and health, please wear a lab coat and disposable gloves while handling the reagents. Show more