IL-27 (Interleukin 27) is a cytokine belonging to the IL-12 family, composed of an EBI3 and a p28 subunit forming a heterodimeric cytokine. IL-27 is produced by activated dendritic cells and macrophages in response to Toll-like receptor ligands and pro-inflammatory cytokines. IL-27 promotes the differentiation of CD4+ T cells into the Th1 lineage by inducing the expression of the transcription factor T-bet and upregulating IL-12Rβ2, which in turn inhibits the differentiation and proliferation of Th2 and Th17 cells. IL-27 is a recently identified member of the pro-inflammatory and immunoregulatory IL-6/IL-12 cytokine family. IL-27 functions by binding to its receptors, WSX-1 and gp130, leading to the activation of the Jak/STAT signaling cascade, indicating that IL-27 is involved in the regulation of immune processes. Recombinant Mouse IL-27 is a non-disulfide-linked heterodimeric protein consisting of a 210-amino acid EBI3 subunit and a 206-amino acid IL-27-p28 subunit. This recombinant product is provided as a lyophilized powder, featuring high activity, high purity, and low endotoxin. Product Information Product Name Product Number Specification Recombinant Mouse IL-27 Protein,His Tag 90371ES10 10 μg 90371ES50 50 μg 90371ES80 1 mg Performance Parameters Synonyms IL-27, h-IL-27, rh-IL-27, IL-27, interleukin-27 Species Mouse Source CHO Cells Sequence Y19-P228&F29-S234 Accession O35228&Q8K3I6 Tag No tag Molecular Weight Predicted molecular mass:48.8 kDa Endotoxin ≤10 EU/mg by the LAL method Purity ≥95%, by SDS-PAGE (under reducing (R) & Non-reducing conditions, visualized by Coomassie staining). Activity Measured in a VSV inhibition assay with Wish cells. The ED50 is ≤1 ng/mL. Formulation PBS, 5% Mannitol and 0.01% Tween 80, pH7.4. Appearance Lyophilized Powder Storage Store at -25~-15°C. Valid for 1 year from the date of receipt. After reconstitution, store at 2~8°C for 7-10 days. After reconstitution, store at -85~-65°C for up to 6 months. Reconstitution Method Reconstitute with sterile deionized water to a concentration of 0.1-1 mg/mL. It is recommended to aliquot the protein upon first use to avoid repeated freeze-thaw cycles. Notes 1. For your safety and health, please wear a lab coat and disposable gloves during operation. 2. This product is for research use only. Documents: Manuals 90371_Manual_HB20260408
