α-Secretase Fluorogenic Substrate Catalog number: B2013153 Lot number: Batch Dependent Expiration Date: Batch dependent Amount: 1 mg Molecular Weight or Concentration: na Supplied as: Powder Applications: molecular tool for various biochemical applications Storage: −20°C Keywords: α-Secretase Substrate II, Fluorogenic, Grade: Biotechnology grade. All products are highly pure. All solutions are made with Type I ultrapure water (resistivity >18 MΩ-cm) and are filtered through 0.22 um. References Choi Y, Cho Y, Kim M, Grailhe R, Song R. Fluorogenic quantum dot-gold nanoparticle assembly for beta secretase inhibitor screening in live cell Anal Chem. 2012 Oct 16;84(20):8595-601. De Simone A, Seidl C, Santos CA, Andrisano V. Liquid chromatographic enzymatic studies with on-line Beta-secretase immobilized enzyme reactor and 4-(4-dimethylaminophenylazo) benzoic acid/5-[(2-aminoethyl) amino] naphthalene-1-sulfonic acid peptide as fluorogenic substrate J Chromatogr B Analyt Technol Biomed Life Sci. 2014 Mar 15;953-954:108-14. Ermolieff J, Loy JA, Koelsch G, Tang J. Proteolytic activation of recombinant pro-memapsin 2 (pro-beta-secretase) studied with new fluorogenic substrates Biochemistry. 2000 Oct 10;39(40):12450-6. Kasturirangan S, Brune D, Sierks M. Promoting alpha-secretase cleavage of beta-amyloid with engineered proteolytic antibody fragments Biotechnol Prog. 2009 Jul-Aug;25(4):1054-63. Cheng XR, Zhou JW, Zhou Y, Cheng JP, Yang RF, Zhou WX, Zhang YX, Yun LH. The green tea polyphenol (2)-epigallocatechin-3-gallate (EGCG) is not a β-secretase inhibitor Bioorg Med Chem Lett. 2012 Feb 1;22(3):1408-14. Zou Z, Chung B, Nguyen T, Mentone S, Thomson B, Biemesderfer D. Linking receptor-mediated endocytosis and cell signaling: evidence for regulated intramembrane proteolysis of megalin in proximal tubule J Biol Chem. 2004 Aug 13;279(33):34302-10. Li HM, Yu SP, Fan TY, Zhong Y, Gu T, Wu WY, Zhao C, Chen Z, Chen M, Li NG, Wang XL. Design, synthesis, and biological activity evaluation of BACE1 inhibitors with antioxidant activity Drug Dev Res. 2020 Apr;81(2):206-214. Wang LF, Zhang R, Xie X. Development of a high-throughput assay for screening of gamma-secretase inhibitor with endogenous human, mouse or Drosophila gamma-secretase Molecules. 2009 Sep 14;14(9):3589-99. Florean C, Zampese E, Zanese M, Brunello L, Ichas F, De Giorgi F, Pizzo P. High content analysis of gamma-secretase activity reveals variable dominance of presenilin mutations linked to familial Alzheimer’s disease Biochim Biophys Acta. 2008 Aug;1783(8):1551-60.
